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Automated colorimetric gadolinium assay for verification of clearance and estimation of glomerular filtration rate.
Clin Chim Acta. 2008 Sep 14;
Authors: Magnotti RA, Connell JL, Marietta PM
BACKGROUND: NSF (nephrogenic systemic fibrosis) is a potentially serious adverse effect for renal patients undergoing MRI (magnetic resonance imaging) procedures using gadolinium-containing contrast agents. There is therefore a need to verify clearance of these agents and to confirm appropriate renal status of patients treated with these drugs. METHODS: Serum samples from canine and feline subjects dosed with 0.1 mmol/kg of gadolinium agent, or from spiked samples were assayed for gadopentetate (Magnevist((R))), gadodiamide (Omniscan((R))) or gadoversetamide (OptiMARK((R))) using a new dye reagent on the Olympus AU400. Accuracy was verified by ICP-MS. RESULTS: The reportable dynamic range is 3-600 micromol/l Gd. Split serum samples from animals dosed with 0.1 mmol/kg of gadopentetate ranged from 7-458 micromol/l Gd: y=1.121x+0.267, r=0.996, for the Olympus method as a function of Gd measurement by ICP-MS. Between-day imprecision was 2.7% CV-2.0% CV for samples ranging from 12-400 micromol/l Gd. CONCLUSIONS: The assay is useful to verify the clearance of gadolinium and for evaluation of renal status by estimation of GFR using gadopentetate.
PMID: 18834869 [PubMed – as supplied by publisher]
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